| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059914 |
| Chromosome: | chromosome 1 |
| Location: | 4826656 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCCGGCGATGGCCACCGCCTCCACCTG |
| Internal bar code: | CGGCTGTGGGCACCCGTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 93.4551 |
| LEAP-Seq n confirming: | 614 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACCGTCTTCCTGATCCA |
| Suggested primer 2: | TAGCTGCCAGTGTCATGAGG |