| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059932 |
| Chromosome: | chromosome 16 |
| Location: | 1147585 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650300 | HTR39,HTR25 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCCAATCGCTTTTGCTTGATGTATTGT |
| Internal bar code: | TCGGTTTAGAGGTTTCAGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 99.6073 |
| LEAP-Seq n confirming: | 3805 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGAAGCTGTCCAGTAGC |
| Suggested primer 2: | GTTCGAGGACACCAACCTGT |