| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059933 |
| Chromosome: | chromosome 1 |
| Location: | 4963174 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034300 | (1 of 1) PF09073 - BUD22 (BUD22) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCATGTGCCAGTTTGGGTCAGGGACGGA |
| Internal bar code: | TTGAAATGGTCGCGTGGCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 99.0469 |
| LEAP-Seq n confirming: | 112343 |
| LEAP-Seq n nonconfirming: | 1081 |
| LEAP-Seq n unique pos: | 127 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACACAAAGCAAAATGG |
| Suggested primer 2: | GTTATTGAGGACGCGCTGAT |