| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.059940 |
| Chromosome: | chromosome 12 |
| Location: | 3782428 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515301 | (1 of 4) PF13515 - Fusaric acid resistance protein-like (FUSC_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTATGCCACTCGCTGCATGCACTGGCTGC |
| Internal bar code: | CTGAGTTCCGGGGGACTGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 62.6992 |
| LEAP-Seq n confirming: | 1259 |
| LEAP-Seq n nonconfirming: | 749 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGGATCTCAGGGTGTCT |
| Suggested primer 2: | GTCCGATACGACACAGCTCA |