Insertion junction: LMJ.RY0402.059955_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.059955
Chromosome:chromosome 11
Location:40481
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467527 (1 of 1) PTHR24286:SF0 - CYTOCHROME P450 61 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTCTGACCCGCGTCCCTCTGCGGCTGCT
Internal bar code:TGTCAATGATCAACCGCACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1269
LEAP-Seq percent confirming:99.6286
LEAP-Seq n confirming:9658
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TACGGGTCCTTCACCATCTC
Suggested primer 2:ATTCAAATTCTGCGTGGACC