| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.059965 |
| Chromosome: | chromosome 5 |
| Location: | 1490899 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243801 | FAP387 | Flagellar Associated Protein 387 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCAGACATCCTTGCAGCCCCCAAGGCT |
| Internal bar code: | ATGGCCATAGTGTAGTCCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 120 |
| LEAP-Seq percent confirming: | 99.5614 |
| LEAP-Seq n confirming: | 227 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGTTGTAGGTTTCTGGC |
| Suggested primer 2: | COULD_NOT_FIND_PRIMER |