| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059970 |
| Chromosome: | chromosome 17 |
| Location: | 3698930 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726950 | (1 of 1) PTHR11042//PTHR11042:SF102 - EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED // INTERFERON-INDUCED, DOUBLE-STRANDED RNA-ACTIVATED PROTEIN KINASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGCTGACACGCGCGTGCCCCTGTCACC |
| Internal bar code: | GACTTTCTCCGTACGGAAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 22 |
| LEAP-Seq percent confirming: | 69.3333 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGCTAAGGAGCAATGCG |
| Suggested primer 2: | CTGGGAAGTGGAAGAGCAAG |