Insertion junction: LMJ.RY0402.059982_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.059982
Chromosome:chromosome 14
Location:1002748
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g614200 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCCTACACAGGGGTGGATATGGCGACTA
Internal bar code:GTGCAGAGCCATCGTCGCTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:811
LEAP-Seq percent confirming:99.4895
LEAP-Seq n confirming:3898
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGATGCATCTCCTGTAA
Suggested primer 2:GTTACACCTTACCTCCGGCA