| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.059990 |
| Chromosome: | chromosome 3 |
| Location: | 1683271 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCACGCGTCCAAGGCATGCCGCACCACA |
| Internal bar code: | ATCCACGGGCTGTTTGCAAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 99.5796 |
| LEAP-Seq n confirming: | 3316 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAATGTGCGACTTGTCT |
| Suggested primer 2: | CGCAGTACCATTTCCAGGTT |