| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.059994 |
| Chromosome: | chromosome 13 |
| Location: | 3800540 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589870 | (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAGAATGAGCCGGAAGGGTTACGTCCC |
| Internal bar code: | GCCTGTTCAACAGGTGGTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 769 |
| LEAP-Seq percent confirming: | 99.5277 |
| LEAP-Seq n confirming: | 2950 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGCTACTGCTGCGACCT |
| Suggested primer 2: | ACCGTCTCTCATGTCCCAAC |