| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060011 |
| Chromosome: | chromosome 7 |
| Location: | 2062697 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325850 | MAW8 | Membrane-associated hydroxyproline-rich glycoprotein 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCTGCCCCTGACCCATACCTATCCCAT |
| Internal bar code: | AATGTCCCTGCGCGACTCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 66.7136 |
| LEAP-Seq n confirming: | 948 |
| LEAP-Seq n nonconfirming: | 473 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTTGGGGATCTAGTAGG |
| Suggested primer 2: | CCTGACGTTTGTGTTTGTGG |