| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.060047 |
| Chromosome: | chromosome 12 |
| Location: | 8585039 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547350 | CYG31 | Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCCTGACCGGCGCGCAACAGGTCGGTT |
| Internal bar code: | GCGGACATTGGTCGCAAATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 98.8705 |
| LEAP-Seq n confirming: | 1138 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGCACCTACATCACTGT |
| Suggested primer 2: | GGCATGGTGTGAAACTGATG |