| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060051 |
| Chromosome: | chromosome 1 |
| Location: | 2619050 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015200 | PDE4 | (1 of 17) 3.1.4.53 - 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGTGGCGTTGTTGTAGGCACAGGGGGC |
| Internal bar code: | ACGTGGTACTTTTACGTGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.4165 |
| LEAP-Seq n confirming: | 5963 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTCACTCTGTCCCACCT |
| Suggested primer 2: | ACGGATGAGACTTGGGTGAC |