| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060059 |
| Chromosome: | chromosome 6 |
| Location: | 3596074 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278122 | (1 of 1) PTHR10504:SF73 - PROTEIN C05C9.1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGATTATTCTCGGAGCAGCAGCGAGGCG |
| Internal bar code: | TTGCTCGCTTCCACTACTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1064 |
| LEAP-Seq percent confirming: | 98.8711 |
| LEAP-Seq n confirming: | 2102 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTTTAGGTGGAGGTGCT |
| Suggested primer 2: | TCCTTGATAGCTTCAGGCGT |