| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060071 |
| Chromosome: | chromosome 7 |
| Location: | 2222003 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327250 | (1 of 3) IPR000104//IPR005135 - Antifreeze protein, type I // Endonuclease/exonuclease/phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGCAGCAACCAGGGGCTTCCTGCCGTT |
| Internal bar code: | TAATGAGTCTATTTCTCGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 71.8376 |
| LEAP-Seq n confirming: | 4140 |
| LEAP-Seq n nonconfirming: | 1623 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATCCCAGCCAGTATGGT |
| Suggested primer 2: | AAGCGCTTACAGAAACGGAA |