| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060103 |
| Chromosome: | chromosome 3 |
| Location: | 8598620 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202897 | UBC34 | E2 ubiquitin conjugating enzyme; (1 of 1) K10578 - ubiquitin-conjugating enzyme E2 J1 (UBE2J1, NCUBE1, UBC6) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGGCCGTTGGGCGTCAGCATCATGAAC |
| Internal bar code: | CAGGATCACGGATGGCCAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 98.2926 |
| LEAP-Seq n confirming: | 5584 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTGTGGACAACCTGCAC |
| Suggested primer 2: | CAGAAGGCGTCTACGGTCTC |