| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060123 |
| Chromosome: | chromosome 13 |
| Location: | 2005953 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576720 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGCCGGCGTCACTGGTTTTGGGACTGC |
| Internal bar code: | GGGGCATAATGGCACGGAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 24.8308 |
| LEAP-Seq n confirming: | 477 |
| LEAP-Seq n nonconfirming: | 1444 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATAACCTTCGCTGCTGT |
| Suggested primer 2: | CAGTGAGTCCTAGCCAAGCC |