| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060128 |
| Chromosome: | chromosome 3 |
| Location: | 8275433 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199250 | CYG51 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCACACCAGCTTACTTGCAACGTGGCC |
| Internal bar code: | GCTGCGGGCATTTCCGCGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 6.8306 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 682 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACCTAGCGAGTTCGTGA |
| Suggested primer 2: | GGCTGGGCACAAGTTACATT |