| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060132 |
| Chromosome: | chromosome 3 |
| Location: | 1160205 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149350 | RWP4 | (1 of 16) PF02042 - RWP-RK domain (RWP-RK); RWP-RK transcription factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCAGGAGCGCCATTGCCGCCGTCGGAG |
| Internal bar code: | GGGTTGGATTTTTGCAACATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 98.4513 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCACATCCATATCGCCTC |
| Suggested primer 2: | ACTGAATTCGTTGCTCACCC |