| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060153 |
| Chromosome: | chromosome 12 |
| Location: | 4297139 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGCTGGTGACCACTGCACACATTCTTG |
| Internal bar code: | TATGACTAAATGATCTACCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1106 |
| LEAP-Seq percent confirming: | 99.4565 |
| LEAP-Seq n confirming: | 1281 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGATCGCGCATCAATCAA |
| Suggested primer 2: | CTGGAAAACCACGTCAGGTT |