| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.060156 |
| Chromosome: | chromosome 12 |
| Location: | 4464684 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521550 | (1 of 1) PF00630//PF02493 - Filamin/ABP280 repeat (Filamin) // MORN repeat (MORN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGTTTTAGGCCGCACGATTACACGCG |
| Internal bar code: | CCTCACCGCCGGGGGGAAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 99.4024 |
| LEAP-Seq n confirming: | 998 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATTCTCACCCGTGTACT |
| Suggested primer 2: | GGATGTGTACAGGTTCGGCT |