| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060157 |
| Chromosome: | chromosome 10 |
| Location: | 5638347 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460300 | PGM2 | (1 of 2) K15634 - probable phosphoglycerate mutase (gpmB); Phosphoglycerate mutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCGGATGGAGGGACACGGCGTGTAAGG |
| Internal bar code: | CTGTTCTTAGTGTTTGGTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 99.7199 |
| LEAP-Seq n confirming: | 6053 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTTGCAGCGAGTTCATC |
| Suggested primer 2: | GGCTTGAAAACGAAGACAGC |