| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060167 |
| Chromosome: | chromosome 9 |
| Location: | 6970814 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410650 | HIS1 | (1 of 1) 2.4.2.17 - ATP phosphoribosyltransferase / Phosphoribosyl-ATP pyrophosphorylase; N-5-phosphoribosyl-ATP transferase | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCCCCCACGCGTGCTGCTCTTAGTCAG |
| Internal bar code: | TGCCTGGTGTAGATCCTCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 95.7187 |
| LEAP-Seq n confirming: | 313 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAACGCAAAATGTGTGTG |
| Suggested primer 2: | TTACCTCCCAGCATTCCAAG |