Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.060169 |
Chromosome: | chromosome_14 |
Location: | 316845 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g609926 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCCTCATGCCCCGTGTCTCACTCGCTCTC |
Internal bar code: | GACTTCAGACTGCCTCAGAAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 322 |
LEAP-Seq percent confirming: | 99.9076 |
LEAP-Seq n confirming: | 1081 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGTGGTGGAGGTGGAG |
Suggested primer 2: | TGCAACTTGCTCTCCAACAC |