Insertion junction: LMJ.RY0402.060175_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.060175
Chromosome:chromosome 5
Location:2638683
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g236400 NAT13 (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGAGGAGGGTTGGAGAAGTAGTACAAG
Internal bar code:ACGTACATTAAGGTTGTCACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:911
LEAP-Seq percent confirming:99.2132
LEAP-Seq n confirming:6179
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAAATGGCATATCCACG
Suggested primer 2:ACACGTAGCTCCCTTGTGCT