| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060183 |
| Chromosome: | chromosome 12 |
| Location: | 9632387 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551977 | PHC74 | Putative pherophorin-chlamydomonas homolog; (1 of 1) 2.7.11.1//3.6.5.5 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dynamin GTPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTGCCAGACATGCAAGGCATCTGGTAA |
| Internal bar code: | AGTTAGTTGTTTGTCGGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 93.8775 |
| LEAP-Seq n confirming: | 460 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTTCAAGCAAGCAAGG |
| Suggested primer 2: | AATTCACCCTTGACTCACCG |