| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060219 |
| Chromosome: | chromosome_8 |
| Location: | 1368004 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g364000 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAAACTGAACAATAGTGCGGCAAATGGTGT |
| Internal bar code: | GGCGGCCTGAGTTGAAAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 99.4773 |
| LEAP-Seq n confirming: | 9325 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGCGCCTGTACATAGCC |
| Suggested primer 2: | AGTTCATGTTGGGTCCAAGG |