| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060223 |
| Chromosome: | chromosome 10 |
| Location: | 5531143 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459450 | SUB10 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCATTGGGCGGGCTTTCGCTTCGTTCT |
| Internal bar code: | AGGAATTTCGCTTTTAAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 96.3898 |
| LEAP-Seq n confirming: | 7369 |
| LEAP-Seq n nonconfirming: | 276 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGATATTGCCAAAACACT |
| Suggested primer 2: | GCATCGTTAAGGCACTGGTT |