| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060223 |
| Chromosome: | chromosome 11 |
| Location: | 1750123 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467792 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAATTTCGGCCAAAACGCACGGGATTTT |
| Internal bar code: | ACCGGACTCAGGAAACATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 144 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTGTACCGCCAATCAGG |
| Suggested primer 2: | GTCATGCGTACGGCTATCCT |