| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060229 |
| Chromosome: | chromosome 2 |
| Location: | 1199632 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082000 | AGE1 | DNA repair glycosylase; (1 of 1) K03575 - A/G-specific adenine glycosylase (mutY) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCTACGGTAGCAATGCGCAAGTACACG |
| Internal bar code: | ACACAAAGTAGGACGACAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 99.1262 |
| LEAP-Seq n confirming: | 1021 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGTCGCTGACAACCGTG |
| Suggested primer 2: | ACGATGCAGGACTTTGCTCT |