| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.060238 |
| Chromosome: | chromosome 1 |
| Location: | 7456337 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053500 | (1 of 1) K05956 - geranylgeranyl transferase type-2 subunit beta [EC:2.5.1.60] (RABGGTB) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGAACAGGTCTCGGTCCACCAGGTGCAG |
| Internal bar code: | CTGTCCACATCAATACGGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 95.2401 |
| LEAP-Seq n confirming: | 2221 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTATTGGGTTGATTCCA |
| Suggested primer 2: | TCACCCTTTAGACGGTACGC |