Insertion junction: LMJ.RY0402.060253_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.060253
Chromosome:chromosome 3
Location:3852157
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g170800 (1 of 1) PTHR12231//PTHR12231:SF138 - CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACAGTTCTTAAATCCCCGTGCCCACTCG
Internal bar code:TGCGGTCTAAGTACCCACGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:389
LEAP-Seq percent confirming:99.645
LEAP-Seq n confirming:1965
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGACCAGTGACCACCGTTTC
Suggested primer 2:TTTCCAAGGTGTTTGGCTTC