| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060253 |
| Chromosome: | chromosome 3 |
| Location: | 3852161 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170800 | (1 of 1) PTHR12231//PTHR12231:SF138 - CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTGAGGAAAGGCTTCATCGCCATCATCG |
| Internal bar code: | CGGACCCTCAAATTGCCTTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 897 |
| LEAP-Seq percent confirming: | 99.2837 |
| LEAP-Seq n confirming: | 1386 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCAAGGTGTTTGGCTTC |
| Suggested primer 2: | AGACCAGTGACCACCGTTTC |