| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060270 |
| Chromosome: | chromosome 11 |
| Location: | 1820414 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467796 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTATTCCTGTTGGGTTTTTCAACACGC |
| Internal bar code: | GGAAGATAATCGGGCAACGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1041 |
| LEAP-Seq percent confirming: | 98.9994 |
| LEAP-Seq n confirming: | 5145 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCGTAACAGGGAAATAC |
| Suggested primer 2: | GAGAGGTGACAAGCGGAAAG |