| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060289 |
| Chromosome: | chromosome 5 |
| Location: | 1151640 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245701 | FAP104 | Flagellar Associated Protein 104 | 3'UTR|intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATTGTGAGGAGGGAAGGGCTCTGGGAG |
| Internal bar code: | ATCATCCGGTGTCATTGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 99.559 |
| LEAP-Seq n confirming: | 9482 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTCCGTGCATCAAACAC |
| Suggested primer 2: | CGACTACAAACAGCTCACGG |