Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.060289 |
Chromosome: | chromosome_5 |
Location: | 1151640 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g245701 | FAP104 | antisense | 3'UTR|intron|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGAATTGTGAGGAGGGAAGGGCTCTGGGAG |
Internal bar code: | ATCATCCGGTGTCATTGAAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 640 |
LEAP-Seq percent confirming: | 99.559 |
LEAP-Seq n confirming: | 9482 |
LEAP-Seq n nonconfirming: | 42 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAACTCCGTGCATCAAACAC |
Suggested primer 2: | CGACTACAAACAGCTCACGG |