| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060295 |
| Chromosome: | chromosome 7 |
| Location: | 3448786 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335400 | EttA | Putative soluble chloroplast ABCF protein, relatated to bacterial EttA which throttles translation speed; (1 of 1) PTHR19211:SF7 - ABC TRANSPORTER-RELATED PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCACACACGTGCACCTGCGCCTCTGCAG |
| Internal bar code: | GAGGGCGTCGCCGCCGCGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 840 |
| LEAP-Seq percent confirming: | 99.6218 |
| LEAP-Seq n confirming: | 2371 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATCACCACGCACCTTTA |
| Suggested primer 2: | AGACGCACACAGACACAAGG |