| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.060295 |
| Chromosome: | chromosome 7 |
| Location: | 3448787 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335400 | EttA | Putative soluble chloroplast ABCF protein, relatated to bacterial EttA which throttles translation speed; (1 of 1) PTHR19211:SF7 - ABC TRANSPORTER-RELATED PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATGCATACAACTTCTCGTTTAGTTTCC |
| Internal bar code: | AGCCTGGAATGGCATCACGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 894 |
| LEAP-Seq percent confirming: | 97.8406 |
| LEAP-Seq n confirming: | 3670 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGCACACAGACACAAGG |
| Suggested primer 2: | TTGATCACCACGCACCTTTA |