| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060332 |
| Chromosome: | chromosome 7 |
| Location: | 3670416 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337350 | GT90F8,GT90-8 | GT90 family protein 8; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACTGAGATAACGAGCCCGACCGCAGGC |
| Internal bar code: | TTAATGTGAACGTTCGGGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 99.8574 |
| LEAP-Seq n confirming: | 4201 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTGTCCACGCTGTACCC |
| Suggested primer 2: | GGAGAGTCACCTTCCCACAA |