| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.060337 |
| Chromosome: | chromosome 7 |
| Location: | 5142498 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348040 | (1 of 4) PTHR11785//PTHR11785:SF367 - AMINO ACID TRANSPORTER // AMINO-ACID PERMEASE BAT1; Probable amino acid/metabolite permease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCATGCGAATTTGCCAAGCAGCCCTGCA |
| Internal bar code: | GGAAAGCACCGGATTGTGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 320 |
| LEAP-Seq percent confirming: | 81.5867 |
| LEAP-Seq n confirming: | 6057 |
| LEAP-Seq n nonconfirming: | 1367 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAACAGCTAAGAACTGC |
| Suggested primer 2: | AGCAGCAGTAGCAGAGGAGC |