| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.060340 |
| Chromosome: | chromosome 13 |
| Location: | 4531955 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602700 | SPA1 | Part of COP1/SPA complex E3 ubiquitin ligase complex; (1 of 1) K16240 - protein suppressor of PHYA-105 1 (SPA1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCATCCCCAGCTGCGGCCCAAGTCCCAG |
| Internal bar code: | GAGTAGAGGAGGGCGAAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 99.1354 |
| LEAP-Seq n confirming: | 344 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAAAAGCTTTATTGCGCC |
| Suggested primer 2: | CTAAGCTCCGTCACCTGGAG |