| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060346 |
| Chromosome: | chromosome_12 |
| Location: | 6128907 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g536000 | ALA2 | ATPase, aminophospholipid transporter | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATATCTTACCACCTGGCTATGCAGTTCCGT |
| Internal bar code: | CTGGTCGGGCATCGGAGTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 98.6683 |
| LEAP-Seq n confirming: | 3927 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTCACGTGGACCTTACA |
| Suggested primer 2: | CATTGTGTTTGCTGTCTGGG |