| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060347 |
| Chromosome: | chromosome 2 |
| Location: | 1200834 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082000 | AGE1 | DNA repair glycosylase; (1 of 1) K03575 - A/G-specific adenine glycosylase (mutY) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCACAGGGCGGCATGTGTGTGTGTGTG |
| Internal bar code: | ATACTCTGCCAATGATTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1195 |
| LEAP-Seq percent confirming: | 99.7752 |
| LEAP-Seq n confirming: | 1775 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGTCGCATCACAAGAAG |
| Suggested primer 2: | CAGACAGCGGCTTTACATCA |