| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060358 |
| Chromosome: | chromosome 12 |
| Location: | 4832520 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524750 | (1 of 1) PTHR13504:SF29 - FIDO DOMAIN-CONTAINING PROTEIN DDB_G0283145 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGCTTCAAGAACCCCTTCCACGCCGGC |
| Internal bar code: | TCGCGTGCGTTGCGTGACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 99.8122 |
| LEAP-Seq n confirming: | 12758 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCCTCCAAAAGCAACAG |
| Suggested primer 2: | AACCGTAACGACCACTCCTG |