| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.060360 |
| Chromosome: | scaffold 18 |
| Location: | 161750 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749247 | (1 of 6) PTHR10527 - IMPORTIN BETA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTACCTTTCCCCCTTTGCCGCACTGCGG |
| Internal bar code: | AACTTACCGCGGATCTGTATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 96.4728 |
| LEAP-Seq n confirming: | 1012 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAACTTCGTGAGTGCAGGG |
| Suggested primer 2: | CGCATTGAAGGAACAGGTTT |