Insertion junction: LMJ.RY0402.060393_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.060393
Chromosome:chromosome 12
Location:6503433
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g538550 (1 of 21) IPR000679 - Zinc finger, GATA-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCATAGTGATACAAGACGACGGATCGGT
Internal bar code:GCAGACTTCGCGTAGTTCCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:693
LEAP-Seq percent confirming:97.6711
LEAP-Seq n confirming:4068
LEAP-Seq n nonconfirming:97
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AACAACAACAGCAGCACCAG
Suggested primer 2:TACTGGGCGGTACCTTGTTC