| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060408 |
| Chromosome: | chromosome 17 |
| Location: | 7080320 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g747147 | (1 of 14) PF10294 - Lysine methyltransferase (Methyltransf_16) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAGTGATTCCTTGTATCACGTGTAGCAG |
| Internal bar code: | GTGACGCACTGCCCGCCTCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 967 |
| LEAP-Seq percent confirming: | 99.7436 |
| LEAP-Seq n confirming: | 5836 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGTCCACACGATCCCAA |
| Suggested primer 2: | GTAGGCGGCACATTGGTACT |