Insertion junction: LMJ.RY0402.060419_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.060419
Chromosome:chromosome 16
Location:295361
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g694202 (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAACGGGACCTGCTTGATTGAGGTCGGG
Internal bar code:TCCTCGGGATGTCGTGGATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:197
LEAP-Seq percent confirming:97.6744
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGGTACCAGCCCAAACTA
Suggested primer 2:CTGGCTGCTTGTCGTCAATA