| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060420 |
| Chromosome: | chromosome 13 |
| Location: | 1148087 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569600 | (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGCTGCATCGACTCCGAGCACATCATA |
| Internal bar code: | TGTTGTGCTGGATAAAAATTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 98.0769 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGACAGTCCGCTCTTCAC |
| Suggested primer 2: | CGACTGTCCTTGACGTCTGA |