| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060422 |
| Chromosome: | chromosome 16 |
| Location: | 6970154 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677205 | (1 of 4) PF00207 - Alpha-2-macroglobulin family (A2M) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTACGGCAGGGACGATGCGCAGCAAGAAT |
| Internal bar code: | TTTGTTCACAGTCGTCTGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 97.995 |
| LEAP-Seq n confirming: | 1564 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGAACGCCTACGAGGA |
| Suggested primer 2: | TTGCAATGTACCGCATTGTT |