| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060442 |
| Chromosome: | chromosome 3 |
| Location: | 5267726 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183350 | SRH7 | SNF2-related DNA/RNA helicase; (1 of 1) PTHR10799//PTHR10799:SF654 - SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGCACACAGTGCGTTTGGCCATGTGTC |
| Internal bar code: | GTCCAGAATTCATTCAGGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.8264 |
| LEAP-Seq n confirming: | 575 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGAGTCGACGATGAAAT |
| Suggested primer 2: | CACGCTCGGTATTGGTAGGT |